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KMID : 1188320110050020149
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Gut and Liver
2011 Volume.5 No. 2 p.149 ~ p.153
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Differential LINE-1 Hypomethylation of Gastric Low-Grade Dysplasia from High Grade Dysplasia and Intramucosal Cancer
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Lee Jeong-Rok
Chung Woo-Chul
Kim Jin-Dong
Lee Kang-Moon
Paik Chang-Nyol
Jung Sung-Hoon
Jung Ji-Han
Lee Yun-Kyung
Han Sok-Won
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Abstract
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Background/Aims: Gastric epithelial dysplasia is considered a precancerous lesion with a variable clinical course. There is disagreement, however, regarding histology-based diagnoses, which has led to confusion in choosing a therapeutic plan. New objective markers are needed to determine which lesions progress to true malignancy. We measured LINE-1 methylation levels, which have been reported to strongly correlate with the global methylation level in gastric epithelial dysplasia and intramucosal cancer.
Methods: A total of 145 tissue samples were analyzed by two histopathologists. All tissues were excised by therapeutic endoscopic mucosal resection and paired with adjacent normal tissue samples. A modified long interspersed nucleotide elements?ombined bisulfite restriction analysis (COBRALINE- 1) method was used.
Results: Gastric epithelial dysplasia and intramucosal cancer tissues had significantly lower levels of LINE-1 methylation than adjacent normal gastric tissues. Highgrade dysplasia and intramucosal cancer were distinguishable from low-grade dysplasia based on LINE-1 methylation levels. Furthermore, the distinction could be determined with high sensitivity and specificity, as shown by the receiver operating characteristic (ROC) curve (AUC, 0.82; 95% confidence interval, 0.74 to 0.88).
Conclusions: LINE-1 methylation levels may provide a diagnostic tool for identifying high-grade dysplasia and intramucosal cancer.
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KEYWORD
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LINE-1 methylation, Gastric epithelial dysplasia, Intramucosal cancer
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